Standardization of sample preparation, staining and sampling methods for automated sperm head morphometry analysis of boar spermatozoa.

@article{GarcaHerreros2006StandardizationOS,
  title={Standardization of sample preparation, staining and sampling methods for automated sperm head morphometry analysis of boar spermatozoa.},
  author={Manuel Garc{\'i}a-Herreros and In{\'e}s M. Aparicio and Facundo Jorro Bar{\'o}n and L J Garcia-Marin and Maria Cruz Gil},
  journal={International journal of andrology},
  year={2006},
  volume={29 5},
  pages={
          553-63
        }
}
The accuracy of computer-assisted sperm morphometry analysis (CASMA) depends on the careful preparation, fixation and staining of spermatozoa. The efficiency of CASMA may be enhanced by developing optimized protocols. The aim of the present study was to evaluate the influence of sperm washing and the use of three staining techniques [rapid Panoptic, Hemacolor and Harris's Haematoxylin (HH)] on image-processing accuracy and boar sperm head morphometry. Sperm washing had a significant effect on… CONTINUE READING
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