Standardization of real-time PCR gene expression data from independent biological replicates.

  title={Standardization of real-time PCR gene expression data from independent biological replicates.},
  author={Erik Willems and Luc Leyns and Jo Vandesompele},
  journal={Analytical biochemistry},
  volume={379 1},
Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Corrective methods for different steps in the qRT-PCR reaction have been reported; however, statistical analysis and presentation of substantially variable biological repeats present problems and are often not meaningful, for example, in a biological system such as mouse embryonic stem cell differentiation. Based on a series of… CONTINUE READING
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