Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?

@article{Robert2009StandardizationOP,
  title={Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?},
  author={Sacha Robert and Philippe Poncelet and Romaric Lacroix and Laurent Arnaud and Laurent Giraudo and A Hauchard and Jos{\'e} Sampol and Françoise Dignat-George},
  journal={Journal of thrombosis and haemostasis : JTH},
  year={2009},
  volume={7 1},
  pages={190-7}
}
BACKGROUND Platelet microparticles (PMPs) have proved useful to identify patients with vascular risk. However, PMP counting, which is currently done by flow cytometry (FCM), needs to be standardized. OBJECTIVES The objectives were (i) to standardize FCM settings for PMP counts on a routine instrument (Cytomics FC500) using size-calibrated fluorescent beads; (ii) to determine intra-instrument and inter-instrument reproducibility; and (iii) to establish PMP values in healthy subjects. METHODS… CONTINUE READING
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