Standardization of medium for culturing Lyme disease spirochetes.

@article{Pollack1993StandardizationOM,
  title={Standardization of medium for culturing Lyme disease spirochetes.},
  author={Richard J. Pollack and Samuel R Telford and Andrew L Spielman},
  journal={Journal of clinical microbiology},
  year={1993},
  volume={31 5},
  pages={1251-5}
}
To standardize the procedure for isolating and culturing Lyme disease spirochetes, we modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow… CONTINUE READING

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