Standardization of human diploid cell cultivation.

  title={Standardization of human diploid cell cultivation.},
  author={Jack Litwin},
  journal={Applied microbiology},
  volume={20 6},
Human embryonic diploid lung fibroblasts grown in Eagle's medium were exposed continually to a variety of environmental conditions over a large number of passages to observe how these conditions affected the growth and longevity of these cells in vitro. The cells grew well at temperatures between 34 and 37 C and some cells could be adapted to grow at 40 C. Very limited growth occurred at 30 to 31 C; however, confluent monolayers of cells could be maintained for months at 30 C and still give… CONTINUE READING