Stalled Spliceosomes Are a Signal for RNAi-Mediated Genome Defense

  title={Stalled Spliceosomes Are a Signal for RNAi-Mediated Genome Defense},
  author={Phillip A. Dumesic and Prashanthi Natarajan and Changbin Chen and Ines A. Drinnenberg and Benjamin J. Schiller and James Thompson and James J. Moresco and John R. Yates and David P. Bartel and Hiten D Madhani},
Using the yeast Cryptococcus neoformans, we describe a mechanism by which transposons are initially targeted for RNAi-mediated genome defense. We show that intron-containing mRNA precursors template siRNA synthesis. We identify a Spliceosome-Coupled And Nuclear RNAi (SCANR) complex required for siRNA synthesis and demonstrate that it physically associates with the spliceosome. We find that RNAi target transcripts are distinguished by suboptimal introns and abnormally high occupancy on… CONTINUE READING
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