Staining protocol for organotypic hippocampal slice cultures

  title={Staining protocol for organotypic hippocampal slice cultures},
  author={Nadine Gogolla and Ivan Galimberti and Vincenzo DePaola and Pico Caroni},
  journal={Nature Protocols},
This protocol details a method to immunostain organotypic slice cultures from mouse hippocampus. The cultures are based on the interface method, which does not require special equipment, is easy to execute and yields slice cultures that can be imaged repeatedly, from the time of isolation at postnatal day 6–9 up to 6 months in vitro. The preserved tissue architecture facilitates the analysis of defined hippocampal synapses, cells and entire projections. Time-lapse imaging is based on transgenes… CONTINUE READING


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