Staining paraffin sections without prior removal of the wax.

  title={Staining paraffin sections without prior removal of the wax.},
  author={J A Kiernan},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  volume={71 6},
  • J A Kiernan
  • Published 1996 in
    Biotechnic & histochemistry : official…
Paraffin sections are usually rehydrated before staining. It is possible to apply aqueous dye solutions without first removing the wax. Staining then occurs more slowly, and only if the embedding medium has not melted or become unduly soft after cutting. To avoid this problem, sections are flattened on water no hotter than 45 C and dried overnight at 40 C… CONTINUE READING