Staining paraffin extracted, alcohol rinsed and air dried plant tissue with an aqueous mixture of three dyes.

@article{Graham1998StainingPE,
  title={Staining paraffin extracted, alcohol rinsed and air dried plant tissue with an aqueous mixture of three dyes.},
  author={Effin T. Graham and W. Robert Trentham},
  journal={Biotechnic \& histochemistry : official publication of the Biological Stain Commission},
  year={1998},
  volume={73 4},
  pages={
          178-85
        }
}
  • E. T. Graham, W. R. Trentham
  • Published 1 July 1998
  • Chemistry, Medicine
  • Biotechnic & histochemistry : official publication of the Biological Stain Commission
A staining solution containing alcian blue 8GX, Bismarck brown Y and safranin O was prepared with 0.1 M sodium acetate buffer, pH 5.0. Paraffin was extracted with MicroClear solvent from 10 microm tissue sections mounted on slides. Paraffin solvent was removed by rinsing with isopropanol, and tissues were air dried. Slides with bare dry tissue sections were immersed in the triple stain and structures could be distinguished within 30 min as follows: nonlignified cell walls, blue; lignified cell… 
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The traditional paraffin microtechnique has been modified at all steps from fixation to finishing slides with coverslips, especially for onion root tips fixed in a proprietary solution without aldehyde, toxic metals or acetic acid.
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    Biotechnic & histochemistry : official publication of the Biological Stain Commission
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