Staining methods for magnetic resonance microscopy of the rat fetus.

@article{Petiet2007StainingMF,
  title={Staining methods for magnetic resonance microscopy of the rat fetus.},
  author={Alexandra E Petiet and Laurence W. Hedlund and G. Allan Johnson},
  journal={Journal of magnetic resonance imaging : JMRI},
  year={2007},
  volume={25 6},
  pages={1192-8}
}
PURPOSE To develop a magnetic resonance histology (MRH) staining and fixation method by immersion to enhance the signal-to-noise ratio (SNR) with a paramagnetic contrast agent permitting microscopic acquisition within a 3-hour scan time. MATERIALS AND METHODS Methods were optimized for embryonic day 18.5 (E18.5) rat fetuses and imaging at 9.4T with an RF refocused spin-echo pulse sequence (TR/TE = 75 msec/5.2 msec). Fixation/staining was performed by immersion in Bouin's fixative containing… CONTINUE READING