Stained Pectin as Seen in the Electron Microscope

@article{Albersheim1960StainedPA,
  title={Stained Pectin as Seen in the Electron Microscope},
  author={Peter Albersheim and Kurt M{\"u}hlethaler and Albert Frey-Wyssling},
  journal={The Journal of Biophysical and Biochemical Cytology},
  year={1960},
  volume={8},
  pages={501 - 506}
}
This paper describes electron microscopic studies on the distribution of pectin within young plant cells. Dark-grown onion roots, from 1 to 3 mm. in length, were used. In order to make the pectic substances selectively dense to electrons, they were first reacted with basic hydroxylamine. This treatment produces pectic hydroxamic acids, which in turn were treated with ferric ion to form insoluble complexes. The tissue was imbedded, sectioned, and then observed by electron microscopy. Dense… CONTINUE READING