Stages of embryonic development in the amphipod crustacean, Parhyale hawaiensis

  title={Stages of embryonic development in the amphipod crustacean, Parhyale hawaiensis},
  author={William E. Browne and Alivia Lee Price and Matthias Gerberding and Nipam H. Patel},
Studying the relationship between development and evolution and its role in the generation of biological diversity has been reinvigorated by new techniques in genetics and molecular biology. However, exploiting these techniques to examine the evolution of development requires that a great deal of detail be known regarding the embryonic development of multiple species studied in a phylogenetic context. Crustaceans are an enormously successful group of arthropods and extant species demonstrate a… 

The crustacean Parhyale hawaiensis: a new model for arthropod development.

Bacterial artificial chromosome clones containing genes of interest to the developmental and evolutionary biology communities are being targeted for sequencing, and an expressed sequence tag database will facilitate discovery of additional developmental genes and should broaden the understanding of the genetic controls of body patterning.

The embryonic development of the malacostracan crustacean Porcellio scaber (Isopoda, Oniscidea)

The purpose of these studies is to offer an easy way to define the different degrees of development for future comparative analyses of embryonic development amongst crustaceans in particular, as well as between different arthropod groups.

hawaiensis ( Dana , 1853 )

Considering various established protocols for analyzing and manipulating P. hawaiensis, this organism is a suitable model to gain deeper understanding of brain anatomy e.g. by using connectome approaches, and this study can serve as first solid basis for following studies.

Fixation and dissection of Parhyale hawaiensis embryos.

This protocol describes the dissection and fixation of P. hawaiensis embryos and findsEmbryonic tissue fixed in the following manner is suitable for in situ hybridization experiments to study mRNA expression or for immunocytochemistry to study protein localization.

Studies on development in Euphilomedes ostracods: Embryology, nervous system development, and the genetics of sexually dimorphic eye development

The main events in Euphilomedes’ embryology are studied, focusing on cleavage and cell migration, and a visual staging scheme for their development is described.

Ontogenetic Development of Porcellio scaber: Staging Based on Microscopic Anatomy

A staging system of P. scaber embryos and marsupial mancas based on morphological observations with light microscopy techniques should provide a useful basis for the expanding field of gene expression studies during arthropod embryogenesis and enable comparative developmental studies betweenArthropod groups.

Cellular interactions during gastrulation in the amphipod crustacean, Parhyale hawaiensis

This study finds cell shape change in the rosette that confirms previous hypotheses suggesting that gastrulation in Parhyale proceeds through ingression or invagination, and finds that the ro Sette and epithelial sheet act autonomously, and that the descendants of different lineages within the rosets are able to internalize independently.

The Crustacean Model Parhyale hawaiensis

The marine amphipod Parhyale hawaiensis is currently the best established crustacean system, offering year-round accessibility to developmental stages, transgenic tools, genomic resources, and established genetics and imaging approaches.

Myogenesis of Malacostraca – the “egg-nauplius” concept revisited

It is argued that much of the developmental sequences of larva muscle patterning were retained in the eumalacostracan lineage despite the reduction of free swimming nauplius larvae, but was severely reduced in the peracaridean clade.

The amphipod crustacean Parhyale hawaiensis: An emerging comparative model of arthropod development, evolution, and regeneration

This study introduces Parhyale development and life history, a catalog of techniques and resources for Par Hyale research, and two case studies illustrating its power as a comparative research system.



Molecular genetics of crustacean feeding appendage development and diversification.

Recent studies have used the genetic and molecular data from Drosophila development to try to understand the molecular basis for some of the variations seen in crustacean limbs, and some of these data are reviewed based on the expression patterns of the genes Ultrabithorax, abdominal - A, Sex combs reduced, and Distal-less.

How far does cell lineage influence cell fate specification in crustacean embryos?

The homology of early differentiating neurons in crustaceans and insects seems to be well established and similarities in the expression patterns of the engrailed gene are likely to be homologous and point to a close relationship between these two groups.

Cell lineage analysis of the amphipod crustacean Parhyale hawaiensis reveals an early restriction of cell fates

These findings demonstrate for the first time a total cleavage pattern in an arthropod which results in an invariant cell fate of the blastomeres, but notably, the cell lineage pattern of Parhyale hawaiensis shows no clear resemblance to those found in spiralians, nematodes or deuterostomes.

Cleavage, gastrulation, and germ disc formation of the amphipod Orchestia cavimana (Crustacea, Malacostraca, Peracarida)

The cleavage pattern of the amphipod crustacean Orchestia cavimana from the zygote to gastrulation and the formation of the germ disc is described using direct observation, scanning electron microscopy, histology, video recording, and lineage tracing with a vital dye to contribute to a complete understanding of the embryonic cell lineage of amphipods from the egg to segment formation and organogenesis.

Limb ontogeny and trunk segmentation in Nebalia species (Crustacea, Malacostraca, Leptostraca)

The presence of a row of ventral, sternitic, triangular processes between the bases of the thoracopods, as they are found in certain stages of a species of Nebalia, is suggested as a possible ground pattern for the Malacostraca.

Ribosomal DNA phylogeny of the major extant arthropod classes and the evolution of myriapods

Using nuclear ribosomal gene sequences for constructing a molecular phylogeny, this work provides strong evidence that the crustaceans and not the myriapods should be considered to be the sister group of the insects.

Patterning of the branched head appendages in Schistocerca americana and Tribolium castaneum

The expression patterns of wg, dpp, and Dll in the development of the branched gnathal appendages of the grasshopper, Schistocerca americana, and the flour beetle are detailed to suggest a role for Dpp in forming furrows between tissues.

Structure and function of Halice hesmonectes (Amphipoda: Pardaliscidae) swarms from hydrothermal vents in the eastern Pacific

The results demonstrated that swarms contained a wide range of growth stages, but that small juveniles and mature males and females were absent, and suggested that swarming was not associated with reproductive activity.

Quantitative staging of embryonic development of the grasshopper, Schistocerca nitens.

A system comprising subdivision of embryogenesis into equal periods, expression of stage in percent of complete embryogenesis time, characterization of stages by light micrographs (and descriptive test), and illustration of stages at the egg, embryo, and limb levels of resolution is presented.