Stages in ELISA.

  • Published 2009 in Methods in molecular biology


This chapter gives general information on essential practical features of ELISAs. These can be summarized as follows: 1. Adsorption of antigen or antibody to the plastic solid phase. 2. Addition of the test sample and subsequent reagents. 3. Incubation of reactants. 4. Separation of bound and free reactants by washing. 5. Addition of enzyme-labeled reagent… (More)
DOI: 10.1007/978-1-60327-254-4_3