Stage-specific gut proteinases of the cotton stainer bug Dysdercus peruvianus: role in the release of entomotoxic peptides from Canavalia ensiformis urease.

@article{Piovesan2008StagespecificGP,
  title={Stage-specific gut proteinases of the cotton stainer bug Dysdercus peruvianus: role in the release of entomotoxic peptides from Canavalia ensiformis urease.},
  author={Angela Regina Piovesan and Fernanda Stanisçuaski and Juliana Marco-Salvadori and Rafael Real-Guerra and Marina S. Defferrari and C{\'e}lia Regina Carlini},
  journal={Insect biochemistry and molecular biology},
  year={2008},
  volume={38 11},
  pages={1023-32}
}
Canavalia ensiformis ureases are toxic to insects of different orders. The entomotoxicity of urease is due to a 10 kDa internal peptide released by proteinases in the insect digestive tract. We previously observed that, given orally, urease is toxic to nymphs of Dysdercus peruvianus, but does not affect adults. Here we characterized the major proteolytic activities of D. peruvianus midgut homogenates and investigated their in vitro-catalyzed release of the 10 kDa entomotoxic peptide from urease… CONTINUE READING

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