Stage-specific gene expression during rat spermatogenesis: application of the mRNA differential display method.

@article{Geisinger1996StagespecificGE,
  title={Stage-specific gene expression during rat spermatogenesis: application of the mRNA differential display method.},
  author={A Geisinger and Rodolfo M Wettstein and R. Cabello Benavente},
  journal={The International journal of developmental biology},
  year={1996},
  volume={40 1},
  pages={385-8}
}
Spermatogenesis is a complex differentiation process which requires the coordinate synthesis of diverse stage-specific proteins. In attempting a large-scale identification and characterization of those proteins, we have made use of the recently described mRNA differential display method (Liang and Pardee, Science 257: 967-971, 1992). This method is based on the reverse transcription of mRNAs obtained from two different cell populations (pachytene spermatocytes and spermatids in the present… CONTINUE READING