Stage-specific expressed sequence tags obtained during preimplantation bovine development by differential display RT-PCR and suppression subtractive hybridization.

@article{Ponsuksili2002StagespecificES,
  title={Stage-specific expressed sequence tags obtained during preimplantation bovine development by differential display RT-PCR and suppression subtractive hybridization.},
  author={Siriluck Ponsuksili and David Tesfaye and Nermin El-Halawany and Karl Schellander and Klaus Wimmers},
  journal={Prenatal diagnosis},
  year={2002},
  volume={22 12},
  pages={1135-42}
}
Differential display RT-PCR (DDRT-PCR) and suppression subtractive hybridization (SSH) were applied in order to detect preimplantation stage-specific expressed sequence tags (ESTs) of bovine embryos. Seventeen ESTs were detected from the differential display RT-PCR approach. All clones but two showed homology to genes or ESTs known in human, cattle or other species. One of the clones similar to H. sapiens mRNA for KIAA1764 protein was used exemplarily to quantify the transcripts by real-time… CONTINUE READING

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