Stacking interaction in the middle and at the end of a DNA helix studied with non-natural nucleotides.

  title={Stacking interaction in the middle and at the end of a DNA helix studied with non-natural nucleotides.},
  author={Shu-Ichi Nakano and Hirohito Oka and Yuuki Uotani and Kazuya Uenishi and Masayuki Fujii and Naoki Sugimoto},
  journal={Molecular bioSystems},
  volume={6 10},
Base stacking is important for the base pair interaction of a DNA duplex, DNA replication by polymerases, and single-stranded nucleotide overhangs. To study the mechanisms responsible for DNA stacking interactions, we measured the thermal stability of DNA duplexes containing a non-natural nucleotide tethered to a simple aromatic hydrocarbon group devoid of dipole moments and hydrogen bonding sites. The duplexes containing tetrahydrofuran were paired with a deoxyadenosine derivative (A/T base… Expand
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