Stable wheat transformation obtained without selectable markers

  title={Stable wheat transformation obtained without selectable markers},
  author={Hugo R. Permingeat and Maria Lucrecia Alvarez and Gerardo D. L. Cervigni and Ricardo A. Ravizzini and Rub{\'e}n H. Vallejos},
  journal={Plant Molecular Biology},
Transgenic wheat plants without the selectable marker gene were obtained either in the presence or in the absence of selective pressure during the transformation protocol. When using hygromycin as selective agent in a co-transformation experiment involving a mixture of plasmids pGL2, containing the hpt gene, and pAI1Gus, containing the uidA gene, 3 out of 19 transgenic wheat plants had the uidA gene alone as shown by Southern blots. The gene was transmitted to the progeny following Mendelian… CONTINUE READING
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