Stable transformation of Phaseolus vulgaris via electric-discharge mediated particle acceleration

@article{Russell1993StableTO,
  title={Stable transformation of Phaseolus vulgaris via electric-discharge mediated particle acceleration},
  author={David R. Russell and Ken M. Wallace and J. H. Bathe and Brian J. Martinell and Dennis E. McCabe},
  journal={Plant Cell Reports},
  year={1993},
  volume={12},
  pages={165-169}
}
Transgenic Phaseolus vulgaris or common bean has been produced using electric-discharge particle acceleration. The method uses particle acceleration to introduce DNA into bean seed meristems. Multiple shoots are then generated and screened to recover transgenic plants at a rate of 0.03% germline transformed plants/shoot. We have been able to recover transgenic plants using both GUS and herbicide screening to introduce the gus, bar, and bean golden mosaic virus coat protein genes into the navy… CONTINUE READING

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Plant Cell Rep 9 : 590 - 594 . McCown and Lloyd ( 1981 ) . Proc . International Plant Propagation Soc

  • B McCown, D McCabe, D Russell, D Robison, K Barton, K Raffa
  • 1991

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