Stable transformation and cloning mediated by piggyBac vector and RNA interference knockdown of Drosophila ovarian cell line

@article{Uetake2011StableTA,
  title={Stable transformation and cloning mediated by piggyBac vector and RNA interference knockdown of Drosophila ovarian cell line},
  author={Hiroshi Uetake and Kenji Oka and Yuzo Niki},
  journal={In Vitro Cellular & Developmental Biology - Animal},
  year={2011},
  volume={47},
  pages={689-694}
}
An in vitro study is a powerful method for elucidating gene functions in cellular and developmental events. However, until date, no reliable in vitro transformation, cloning, or knockdown system has been reported for Drosophila cells, with the exception of S2 and Kc cells. In this study, we demonstrated that the piggyBac vector stably integrates donor DNA into ovarian somatic sheets derived from follicle stem cells. The transformed ovarian somatic sheet cells were easily cloned with a new… CONTINUE READING