Stable transfection of retinoblastoma gene promotes contact inhibition of cell growth and hepatocyte nuclear factor-1-mediated transcription in human hepatoma cells.

@article{Awazu1998StableTO,
  title={Stable transfection of retinoblastoma gene promotes contact inhibition of cell growth and hepatocyte nuclear factor-1-mediated transcription in human hepatoma cells.},
  author={S Awazu and Koh Nakata and D Hida and Toshiaki Sakamoto and Kazuhiro Nagata and Nobuko Ishii and Takashi Kanematsu},
  journal={Biochemical and biophysical research communications},
  year={1998},
  volume={252 1},
  pages={269-73}
}
The retinoblastoma (Rb) gene is a key factor involved in cell cycle regulation. In the present study, alterations in cell growth and expression of the alpha-fetoprotein and albumin genes by the stable transfection of the Rb gene were analyzed in HuH-7 human hepatoma cells. Cell growth was almost similar in the Rb gene-transfected and nontransfected cells at the sparse cell density. However, under a confluent condition, contact inhibition of cell growth was stimulated by the Rb gene transfection… CONTINUE READING