Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells

@article{Tonetti2003StableTO,
  title={Stable transfection of an estrogen receptor beta cDNA isoform into MDA-MB-231 breast cancer cells},
  author={Debra A Tonetti and Robyn Rubenstein and Michael Deleon and Huiping Zhao and Virgil Craig Jordan},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  year={2003},
  volume={87},
  pages={47-55}
}
We previously reported stable transfection of estrogen receptor alpha (ERalpha) into the ER-negative MDA-MB-231 cells (S30) as a tool to examine the mechanism of action of estrogen and antiestrogens [J. Natl. Cancer Inst. 84 (1992) 580]. To examine the mechanism of ERbeta action directly, we have similarly created ERbeta stable transfectants in MDA-MB-231 cells. MDA-MB-231 cells were stably transfected with ERbeta cDNA and clones were screened by estrogen response element (ERE)-luciferase assay… CONTINUE READING
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