Stable transduction of the interleukin-2 gene into human natural killer cell lines and their phenotypic and functional characterization in vitro and in vivo.

@article{Nagashima1998StableTO,
  title={Stable transduction of the interleukin-2 gene into human natural killer cell lines and their phenotypic and functional characterization in vitro and in vivo.},
  author={Shigeki Nagashima and Robbie B. Mailliard and Yoshiro Kashii and Torsten Eugen Reichert and Ronald B. Herberman and Paul R. Robbins and Theresa L Whiteside},
  journal={Blood},
  year={1998},
  volume={91 10},
  pages={3850-61}
}
A variety of strategies have been attempted in the past to stably transduce natural killer (NK) cells with cytokine or other cellular genes. Here, we demonstrate the successful delivery of the interleukin-2 (IL-2) gene into two human NK cell lines, IL-2-dependent NK-92 and IL-2-independent YT, by retroviral transduction. An MuLV-based retroviral vector expressing human IL-2 and neor markers from a polycistronic message was constructed and transduced into a CRIP packaging cell line. By… CONTINUE READING

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TL: Human gastric carcinoma transduced with the IL-2 gene: Increased sensitivity to immune effector cells in vitro and in vivo

S Nagashima, Y Kashii, +3 authors Whiteside
Int J Cancer 72:174, • 1997

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