Stable polymers of the axonal cytoskeleton: the axoplasmic ghost

@article{Morris1982StablePO,
  title={Stable polymers of the axonal cytoskeleton: the axoplasmic ghost},
  author={John R. Morris and R. J. Lasek},
  journal={The Journal of Cell Biology},
  year={1982},
  volume={92},
  pages={192 - 198}
}
We have examined the monomer-polymer equilibria which form the cytoskeletal polymers in squid axoplasm by extracting protein at low concentrations of monomer. The solution conditions inside the axon were matched as closely as possible by the extraction buffer (buffer P) to preserve the types of protein associations that occur in axoplasm. Upon extraction in buffer P, all of the neurofilament proteins in axoplasm remain polymerized as part of the stable neurofilament network. In contrast, most… CONTINUE READING
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