Stable isotope dilution analysis of cystine in granulocyte suspensions as cysteine: a powerful method for the diagnosis, the follow-up, and treatment of patients with cystinosis.

@article{Schierbeek1990StableID,
  title={Stable isotope dilution analysis of cystine in granulocyte suspensions as cysteine: a powerful method for the diagnosis, the follow-up, and treatment of patients with cystinosis.},
  author={H H Schierbeek and K. Bijsterveld and Thomas E Chapman and Wilma H J van Luijk and Dirk-Jan Reijngoud and R. G. Berger},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1990},
  volume={191 1-2},
  pages={39-47}
}
A stable isotope dilution method was developed for the determination of cystine in granulocytes. Granulocytes were isolated from blood samples of treated cystinosis patients. Cystine in the granulocyte suspension was decoupled from proteins and converted to cysteine by treatment with a tri-butyl phosphine solution. Tertiary butyldimethyl silyl derivatives were prepared and analyzed by gas chromatography/mass spectrometry. Selective ion monitoring was carried out at m/z 304.3 (M-159 and m/z 406… CONTINUE READING

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