Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.

@article{Eid1991StableIT,
  title={Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.},
  author={Josiane E. Eid and Barbara Sollner-Webb},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 6},
  pages={2118-21}
}
A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants… CONTINUE READING

From This Paper

Topics from this paper.
17 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 17 extracted citations

Similar Papers

Loading similar papers…