Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis

  title={Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis},
  author={Mary-Dell M Chilton and M. Drummond and D. Merlo and D. Sciaky and E. Nester},
  • Mary-Dell M Chilton, M. Drummond, +2 authors E. Nester
  • Published 1977
  • Biology, Medicine
  • Cell
  • Abstract Evidence is presented that crown gall tumors are caused by the incorporation of part of a virulence plasmid carried by the inciting bacterium, Agrobacterium tumefaciens. The rate of reassociation of labeled plasmid DNA was slightly accelerated in the presence of tobacco crown gall tumor DNA, but not normal tobacco DNA. Treatment of tumor DNA with DNAase abolished the acceleration. To determine whether all plasmid sequences are represented in tumor DNA, the labeled plasmid DNA was… CONTINUE READING
    930 Citations
    Tumor DNA structure in plant cells transformed by A. tumefaciens.
    • 200
    The molecular genetics of crown gall tumorigenesis.
    • 113
    Foreign DNA sequences in crown gall teratomas and their fate during the loss of the tumorous traits
    • 16
    Ti-Plasmids: Genetic Engineering of Plants
    • 1


    Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.
    • 584
    • PDF
    Supercoiled circular DNA in crown-gall inducing Agrobacterium strains.
    • 427
    Isolation of covalently closed circular DNA of high molecular weight from bacteria.
    • 289
    Attempts to Detect Agrobacterium tumefaciens DNA in Crown-Gall Tumor Tissue.
    • 18
    • PDF
    The presence of Agrobacterium tumefaciens plasmid DNA in crown gall tumour cells.
    • 24
    • PDF
    Plasmid required for virulence of Agrobacterium tumefaciens.
    • 590
    • PDF
    Evidence for diverse types of large plasmids in tumor-inducing strains of Agrobacterium.
    • 107
    • PDF