Stable implantation of orthogonal sensor circuits in Gram-negative bacteria for environmental release.

@article{Heras2008StableIO,
  title={Stable implantation of orthogonal sensor circuits in Gram-negative bacteria for environmental release.},
  author={Aitor de las Heras and Carlos Arnaud Carre{\~n}o and V{\'i}ctor de Lorenzo},
  journal={Environmental microbiology},
  year={2008},
  volume={10 12},
  pages={
          3305-16
        }
}
A broad host range, orthogonal genetic platform has been developed to format sensor circuits in the chromosome of Gram-negative microorganisms destined for environmental release as bioindicators of toxic or perilous compounds (e.g. explosives) in soil. The genetic scheme includes the generation of a genomic landing pad for the sensor module with a Tn5-mini-transposon bearing an optimal attTn7 sequence and a choice of reporter systems with optical and enzymatic outputs. The array of functional… CONTINUE READING
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