Stable immobilization of rat hepatocytes as hemispheroids onto collagen-conjugated poly-dimethylsiloxane (PDMS) surfaces: importance of direct oxygenation through PDMS for both formation and function.

@article{Nishikawa2008StableIO,
  title={Stable immobilization of rat hepatocytes as hemispheroids onto collagen-conjugated poly-dimethylsiloxane (PDMS) surfaces: importance of direct oxygenation through PDMS for both formation and function.},
  author={Masaki Nishikawa and Takatoki Yamamoto and Nobuhiko Kojima and Komori Kikuo and Teruo Fujii and Yasuyuki Sakai},
  journal={Biotechnology and bioengineering},
  year={2008},
  volume={99 6},
  pages={1472-81}
}
The highly oxygen-permeable material, poly-dimethylsiloxane (PDMS), has the potential to be applied to cell culture microdevices, but cell detachment from PDMS has been a major problem. In this study, we demonstrate that a combination of collagen covalently immobilized PDMS and an adequate oxygen supply enables the establishment of a stable, attached spheroid (hemispheroid) culture of rat hepatocytes. The bottom PDMS surfaces were first treated with oxygen plasma, then coupled with aminosilane… CONTINUE READING