Stable high-level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions.

@article{Butaye2004StableHT,
  title={Stable high-level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions.},
  author={Katleen M. J. Butaye and Inge J. W. M. Goderis and Piet F. J. Wouters and Jonathan M-T G Pues and Stijn L. Delaur{\'e} and Willem F. Broekaert and A. G. Depicker and Bruno P. A. Cammue and Miguel F C De Bolle},
  journal={The Plant journal : for cell and molecular biology},
  year={2004},
  volume={39 3},
  pages={440-9}
}
Basic and applied research involving transgenic plants often requires consistent high-level expression of transgenes. However, high inter-transformant variability of transgene expression caused by various phenomena, including gene silencing, is frequently observed. Here, we show that stable, high-level transgene expression is obtained using Arabidopsis thaliana post-transcriptional gene silencing (PTGS) sgs2 and sgs3 mutants. In populations of first generation (T1) A. thaliana plants… CONTINUE READING
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