Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit

  title={Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit},
  author={S. Ruf and M. Hermann and I. J. Berger and H. Carrer and R. Bock},
  journal={Nature Biotechnology},
  • S. Ruf, M. Hermann, +2 authors R. Bock
  • Published 2001
  • Biology, Medicine
  • Nature Biotechnology
  • Transgenic chloroplasts offer unique advantages in plant biotechnology, including high-level foreign protein expression, absence of epigenetic effects, and gene containment due to the lack of transgene transmission through pollen. [...] Key Result We show that chromoplasts in the tomato fruit express the transgene to ∼50% of the expression levels in leaf chloroplasts. Given the generally very high foreign protein accumulation rates that can be achieved in transgenic chloroplasts (>40% of the total soluble…Expand Abstract
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