Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit

@article{Ruf2001StableGT,
  title={Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit},
  author={S. Ruf and M. Hermann and I. J. Berger and H. Carrer and R. Bock},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={870-875}
}
  • S. Ruf, M. Hermann, +2 authors R. Bock
  • Published 2001
  • Medicine, Biology
  • Nature Biotechnology
  • Transgenic chloroplasts offer unique advantages in plant biotechnology, including high-level foreign protein expression, absence of epigenetic effects, and gene containment due to the lack of transgene transmission through pollen. [...] Key Result We show that chromoplasts in the tomato fruit express the transgene to ∼50% of the expression levels in leaf chloroplasts. Given the generally very high foreign protein accumulation rates that can be achieved in transgenic chloroplasts (>40% of the total soluble…Expand Abstract
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    References

    SHOWING 1-10 OF 33 REFERENCES
    Genetic engineering of the chloroplast.
    • 82
    Technical Advance: Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker.
    • 335
    Stable transformation of plastids in higher plants.
    • 580
    • PDF
    High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
    • Z. Sváb, P. Maliga
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1993
    • 722
    • PDF
    High-yield production of a human therapeutic protein in tobacco chloroplasts
    • 464
    • PDF
    Plastid gene expression during fruit ripening in tomato
    • 75
    • PDF