Stable expression of nephrin and localization to cell-cell contacts in novel murine podocyte cell lines.

@article{Schiwek2004StableEO,
  title={Stable expression of nephrin and localization to cell-cell contacts in novel murine podocyte cell lines.},
  author={Daniel Schiwek and Nicole Endlich and Lawrence B. Holzman and Harry Holth{\"o}fer and Wilhelm Kriz and Karlhans Endlich},
  journal={Kidney international},
  year={2004},
  volume={66 1},
  pages={91-101}
}
BACKGROUND Cell culture of podocytes has become an indispensable tool in the study of podocyte biology. To date, however, podocyte cell lines with stable expression of the crucial slit diaphragm protein nephrin and localization of nephrin to cell-cell contacts are not available. METHODS Conditionally immortalized cells were grown from isolated glomeruli of mice, harboring the temperature-sensitive SV40 large T antigen. About 60 clonal cell lines were generated by limiting dilution. RESULTS… CONTINUE READING
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