Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells

Abstract

Cultured mammalian cells have been a popular and useful system for studying mitosis1–3. In particular, the direct observations of fluorescently tagged cytoskeletal and chromosomal proteins in live cells have furthered our understanding of the dynamics of cell division4–6. Although cultured cells are not suitable for manipulation using genetic methods, they… (More)
DOI: 10.1038/nmeth1205-981

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Cite this paper

@article{Wadsworth2005StableEO, title={Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells}, author={Patricia Wadsworth and Nasser M. Rusan and U. Serdar Tulu and Carey J. Fagerstrom}, journal={Nature Methods}, year={2005}, volume={2}, pages={981-987} }