Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence.

  title={Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence.},
  author={H. Gu and S. Wall and G. Rudnick},
  journal={The Journal of biological chemistry},
  volume={269 10},
We have constructed stable cell lines expressing transporters for dopamine (DA), norepinephrine (NE), and serotonin (5-HT) by transfection with cloned cDNAs. The parental LLC-PK1 cell does not express any of these neurotransmitter transporters. Therefore, monoamine transport activities in each of these cell lines are due to the transfected DNA only, allowing comparison in the same background. Drug inhibition profiles for each cell line are distinct and as expected for each transporter. LLC-NET… Expand
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