Stable dimeric assembly of the second membrane-spanning domain of CFTR (cystic fibrosis transmembrane conductance regulator) reconstitutes a chloride-selective pore.

@article{Ramjeesingh2003StableDA,
  title={Stable dimeric assembly of the second membrane-spanning domain of CFTR (cystic fibrosis transmembrane conductance regulator) reconstitutes a chloride-selective pore.},
  author={Mohabir Ramjeesingh and Francisca Ugwu and Canhui Li and Sonja U. Dhani and Ling Jun Huan and Yanchun Wang and Christine E Bear},
  journal={The Biochemical journal},
  year={2003},
  volume={375 Pt 3},
  pages={633-41}
}
Structural information is required to define the molecular basis for chloride conduction through CFTR (cystic fibrosis transmembrane conductance regulator). Towards this goal, we expressed MSD2, the second of the two MSDs (membrane-spanning domains) of CFTR, encompassing residues 857-1158 in Sf9 cells using the baculovirus system. In Sf9 plasma membranes… CONTINUE READING