Stable co-transformation of maize protoplasts with gusA and neo genes

  title={Stable co-transformation of maize protoplasts with gusA and neo genes},
  author={L. Alexander Lyznik and Randy D. Ryan and Steven W. Ritchie and Thomas K. Hodges},
  journal={Plant Molecular Biology},
An efficient co-transformation protocol using polyethylene glycol was developed for Zea mays L. (cv. A188 × BMS) protoplasts isolated from suspension culture cells. Co-transformation was accomplished by using plasmid constructions containing β-glucuronidase (gusA) or neomycin phosphotransferase (neo) gene coding sequences; both were under control of the CaMV 35S promoter. Protoplast culture and transformation conditions were optimized to assure efficient recovery of transformed cells. The… CONTINUE READING


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Co-transformation of non-selectable higher plant genes into Aspergillus nidulans: Transfer of a Vicia faba legumin B gene

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