Stable and unstable transgene integration sites in the human genome: extinction of the Green Fluorescent Protein transgene in K562 cells.

@article{Migliaccio2000StableAU,
  title={Stable and unstable transgene integration sites in the human genome: extinction of the Green Fluorescent Protein transgene in K562 cells.},
  author={Anna Rita Migliaccio and Chikh Bengra and Jianhua Ling and Wenhu Pi and Changgong Li and Sheng Zeng and Meral Keskintepe and Barry Whitney and Massimo Sanchez and Giovanni Ciro Migliaccio and Dorothy Tuan},
  journal={Gene},
  year={2000},
  volume={256 1-2},
  pages={197-214}
}
In gene transfer experiments including gene therapy studies, expression of the integrated transgenes in host cells often declines with time. The molecular basis of this phenomenon is not clearly understood. We have used the Green Fluorescent Protein (GFP) gene as both a selectable marker and a reporter to study long-term transgene integration and expression in K562 cells. Cells transfected with plasmids containing the GFP gene coupled to the HS2 or HS3 enhancer of the human beta-globin Locus… CONTINUE READING

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