Stable allele replacement and unstable non-homologous integration events during transformation of Ascobolus immersus.

@article{Faugeron1989StableAR,
  title={Stable allele replacement and unstable non-homologous integration events during transformation of Ascobolus immersus.},
  author={Godeleine Faugeron and Christophe Goyon and Annie Gr{\'e}goire},
  journal={Gene},
  year={1989},
  volume={76 1},
  pages={109-19}
}
An homologous transformation system for the filamentous fungus Ascobolus immersus has been developed, based on the complementation of a met2 mutation by the wild-type (wt) allele gene encoding homoserine O-transacetylase. Transformation of A. immersus met2 mutants occurs with moderate frequencies (about 50 transformants per microgram input DNA). Analysis of the DNA of the met2+ transformants showed that transformation resulted either in a single integration of the donor DNA into the genome by… CONTINUE READING

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