Stable Polarized Expression of hCAT-1 in an Epithelial Cell Line

  title={Stable Polarized Expression of hCAT-1 in an Epithelial Cell Line},
  author={R. Cariappa and E. Heath-Monnig and T. Furesz and S. G. Kamath and C. Smith},
  journal={The Journal of Membrane Biology},
Our laboratory has recently identified and cloned three cationic amino-acid transporters of human placenta. We have now examined the plasma membrane domain localization and functional expression of one of these transporters, hCAT-1, in a polarized epithelial cell line (MDCK). To facilitate identification of expressed protein we first transferred the hCAT-1 cDNA to a vector with C-terminal green fluorescent protein (GFP). The resultant hCAT-1-CT-GFP fusion protein stimulated L-[3H] lysine uptake… Expand
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