Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

@inproceedings{Leibly2012StabilizingAA,
  title={Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins},
  author={David J. Leibly and Trang Nhu Nguyen Nguyen and Louis T. Kao and Stephen Nakazawa Hewitt and Lynn K. Barrett and Wesley C E Van Voorhis},
  booktitle={PloS one},
  year={2012}
}
Insoluble recombinant proteins are a major issue for both structural genomics and enzymology research. Greater than 30% of recombinant proteins expressed in Escherichia coli (E. coli) appear to be insoluble. The prevailing view is that insolubly expressed proteins cannot be easily solubilized, and are usually sequestered into inclusion bodies. However, we… CONTINUE READING