Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II

  title={Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II},
  author={X. Deup{\'i} and Patricia Edwards and Ankita Singhal and B. Nickle and D. Oprian and G. Schertler and J. Standfuss},
  journal={Proceedings of the National Academy of Sciences},
  pages={119 - 124}
  • X. Deupí, Patricia Edwards, +4 authors J. Standfuss
  • Published 2011
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • G protein-coupled receptors (GPCR) are seven transmembrane helix proteins that couple binding of extracellular ligands to conformational changes and activation of intracellular G proteins, GPCR kinases, and arrestins. Constitutively active mutants are ubiquitously found among GPCRs and increase the inherent basal activity of the receptor, which often correlates with a pathological outcome. Here, we have used the M257Y6.40 constitutively active mutant of the photoreceptor rhodopsin in… CONTINUE READING
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