Stabilization of thymidine phosphorylase from Escherichia coli by immobilization and post immobilization techniques.

Abstract

Homodimeric thymidine phosphorylase from Escherichia coli (TP, E.C. 2.4.2.4) was immobilized on solid support with the aim to have a stable and recyclable biocatalyst for nucleoside synthesis. Immobilization by ionic adsorption on amine-functionalized agarose and Sepabeads(®) resulted in a very high activity recovery (>85%). To prevent undesirable leakage… (More)
DOI: 10.1016/j.enzmictec.2011.03.011

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