Stabilization of lipid/DNA complexes during the freezing step of the lyophilization process: the particle isolation hypothesis.

@article{Allison2000StabilizationOL,
  title={Stabilization of lipid/DNA complexes during the freezing step of the lyophilization process: the particle isolation hypothesis.},
  author={Stephanie D Allison and M. C. Rios De Molina and Thomas J. Anchordoquy},
  journal={Biochimica et biophysica acta},
  year={2000},
  volume={1468 1-2},
  pages={127-38}
}
The instability of nonviral vectors in aqueous suspensions has stimulated an interest in developing lyophilized formulations for use in gene therapy. Previous work has demonstrated a strong correlation between the maintenance of particle size and retention of transfection rates. Our earlier work has shown that aggregation of nonviral vectors typically… CONTINUE READING