Stabilization of lipid/DNA complexes during the freezing step of the lyophilization process: the particle isolation hypothesis.

@article{Allison2000StabilizationOL,
  title={Stabilization of lipid/DNA complexes during the freezing step of the lyophilization process: the particle isolation hypothesis.},
  author={Stephanie D Allison and Mislene de C. Molina and Thomas J. Anchordoquy},
  journal={Biochimica et biophysica acta},
  year={2000},
  volume={1468 1-2},
  pages={
          127-38
        }
}
The instability of nonviral vectors in aqueous suspensions has stimulated an interest in developing lyophilized formulations for use in gene therapy. Previous work has demonstrated a strong correlation between the maintenance of particle size and retention of transfection rates. Our earlier work has shown that aggregation of nonviral vectors typically occurs during the freezing step of the lyophilization process, and that high concentrations of sugars are capable of maintaining particle size… CONTINUE READING
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