Stabilization of flagellar filaments by HAP2 capping

  title={Stabilization of flagellar filaments by HAP2 capping},
  author={Zolt{\'a}n Di{\'o}szeghy and P{\'e}ter Z{\'a}vodszky and Keiichi Namba and Ferenc Vonderviszt},
  journal={FEBS Letters},
Molecular Determinants of Filament Capping Proteins Required for the Formation of Functional Flagella in Gram-Negative Bacteria
The data indicate that the D1 domain of FliD is its sole functionally important domain, and that its flexible coiled coil region comprised of helices at its extreme N- and C-termini controls compatibility with the FliC filament.
Host cell binding of the flagellar tip protein of Campylobacter jejuni
The results indicate that the C. jejuni flagellar tip protein FliD acts as an attachment factor that interacts with cell surface heparan sulfate glycosaminoglycan receptors.
Role of DegQ in differential stability of flagellin subunits in Vibrio vulnificus
It is demonstrated that flagellins’ production is regulated in a biofilm stage-specific manner, via activities of a protease DegQ and a chaperone FlaJ, which determines the motility-dependent, stage- specific development of biofilms.
A theoretical model of Aquifex pyrophilus flagellin: implications for its thermostability
A predictive 3D-structure of FlaA is developed, using the published structure of mesophilic Salmonella typhimurium flagellin (FliC) as a template and analyzed it with respect to possible determinants of thermostability, finding a predominant stabilizing factor in FlaA appears to be increased hydrophobicity.
Biotemplated synthesis of magnetic filaments.
This study demonstrates that biological templating can be used to produce one-dimensional magnetic nanostructures under benign conditions, and that modified flagellar filaments can beused for creating model systems in which crystal nucleation from solution can be experimentally studied.
Role of Flagellin-Homologous Proteins in Biofilm Formation by Pathogenic Vibrio Species
The results demonstrate that V. vulnificus FlaEF, flagellin-homologous proteins (FHPs), are crucial for biofilm formation by directly interacting with the essential determinant for bio film maturation, EPS.
Examination of the mechanism of the interaction between the HAP 2 cap and the flagellar filament
in international journal: Sebestyén A., Gugolya Z., Jakab G., Diószeghy Z., Závodszky P., Vonderviszt F. (2005): The FliH component of the flagellarexport apparatus is a multi-zinc enzyme with
Análisis bioinformático del sistema flagelar de la alphaproteobacteria tipo rickettsia Candidatus Hepatobacter penaei
The results suggest that Candidatus Hepatobacter penaei is a rickettsial bacterium having a flagellar system, which possibly confers motility and take part in the infection process in hosts such as marine crustaceans.


The bacterial flagellar cap as the rotary promoter of flagellin self-assembly.
The growth of the bacterial flagellar filament occurs at its distal end by self-assembly of flagellin transported from the cytoplasm through the narrow central channel. The cap at the growing end is
Domain movements of HAP2 in the cap–filament complex formation and growth process of the bacterial flagellum
The cap–filament complex built with an atomic model of the filament allows a quantitative analysis of the cap domain movements on cap binding and rotation that promotes the efficient self assembly of flagellin during the filament growth process.
Mechanism of self-association and filament capping by flagellar HAP2.
A model is presented to explain the molecular mechanism of capping, in which conformational entropy in the disordered terminal regions moderates the otherwise too tight HAP2-filament interactions to allow insertion of flagellin subunits below the cap.
Assembly characteristics of flagellar cap protein HAP2 of Salmonella: decamer and pentamer in the pH-sensitive equilibrium.
Crosslinking experiments now clearly show that the HAP2 complex is decameric, and a preferential crosslinking within a pentameric unit together with a bipolar feature of the complex particle observed by electron microscopy suggests that the decamer is a bipolar pair of pentamers.
Plugging interactions of HAP2 pentamer into the distal end of flagellar filament revealed by electron microscopy.
The results suggest that the cap of bacterial flagellum is a pentamer with its thin plate exposed to the solvent and the other half plugged into the hole at the distal end of the filament, which is almost twice wider than its central channel.
Flagellar growth in a filament-less Salmonella fliD mutant supplemented with purified hook-associated protein 2.
This experimental system with exogenous HAP2 allowed us to synchronize filament growth; the average rate of filament growth can be estimated by measuring the length of grown filaments at various time periods in electron micrographs.
Crystal structure of CapZ: structural basis for actin filament barbed end capping
The crystal structure of the chicken sarcomeric capping protein CapZ at 2.1 Å resolution shows a striking resemblance between the α and β subunits, so that the entire molecule has a pseudo 2‐fold rotational symmetry.
"Cap" on the tip of Salmonella flagella.