Stabilization of aminoacyl-tRNA synthetases by sephadex and polyacrylamide gels

@inproceedings{Norris1975StabilizationOA,
  title={Stabilization of aminoacyl-tRNA synthetases by sephadex and polyacrylamide gels},
  author={Roger D. Norris and J. Pawelkiewicz},
  year={1975}
}
Abstract Several aminoacyl- t RNA synthetases from the yellow lupin ( Lupinus luteus ) were stabilized against inactivation during storage at 0–4°, by entrapment in Sephadex or Biogel matrices and drying over P 2 O 5 . The degree of stabilization depended on the rate of drying of the gel and the pH of the medium and to a lesser extent on the ionic strength and protein concentration. With the exception of prolyl- t RNA synthetase, a greater stability was achieved with those enzymes which were… CONTINUE READING