Stabilization of a full-length infectious cDNA clone of transmissible gastroenteritis coronavirus by insertion of an intron.

@article{Gonzalez2002StabilizationOA,
  title={Stabilization of a full-length infectious cDNA clone of transmissible gastroenteritis coronavirus by insertion of an intron.},
  author={Jose M. Gonzalez and Zoltan P{\'e}nzes and F. J. Adell Almaz{\'a}n and Enrique Calvo and Luis Enjuanes},
  journal={Journal of virology},
  year={2002},
  volume={76 9},
  pages={4655-61}
}
The stable propagation of a full-length transmissible gastroenteritis coronavirus (TGEV) cDNA in Escherichia coli cells as a bacterial artificial chromosome has been considerably improved by the insertion of an intron to disrupt a toxic region identified in the viral genome. The viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and the intron was efficiently removed during translocation of this RNA to the cytoplasm. The insertion in two different… CONTINUE READING

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