Stabilization and activation of p53 are regulated independently by different phosphorylation events.

@article{Chernov1998StabilizationAA,
  title={Stabilization and activation of p53 are regulated independently by different phosphorylation events.},
  author={Mikhail V. Chernov and Chilakamarti V. Ramana and V. Adler and George R Stark},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 5},
  pages={2284-9}
}
Treatment of mouse or human cells with the protein kinase C (PKC) inhibitors H7 or bisindolylmaleimide I induced an increase in the lifetime of p53, leading to its accumulation. In inhibitor-treated cells, p53 translocated to the nuclei and bound to DNA but was not competent to induce transcription. However, transactivation could be induced by subsequent DNA damage. Phorbol ester, a potent activator of PKC, significantly inhibited the accumulation of p53 after DNA damage. Therefore… CONTINUE READING