Stability of tumor necrosis factor-α during ion exchange chromatography

Abstract

Denaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. The conformational change and accompanying loss of activity of tumor necrosis factor-α during ion exchange chromatography was reversible and was decreased by adding polyethylene glycol 200 at 2 to 5% (v/v) to… (More)
DOI: 10.1023/A:1024019817164

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