Stability of single copy transgene expression in CHOK1 cells is affected by histone modifications but not by DNA methylation.

@article{Spencer2015StabilityOS,
  title={Stability of single copy transgene expression in CHOK1 cells is affected by histone modifications but not by DNA methylation.},
  author={Shawal Spencer and Agustina Gugliotta and Jennifer D K{\"o}nitzer and Hansjoerg Hauser and Dagmar Wirth},
  journal={Journal of biotechnology},
  year={2015},
  volume={195},
  pages={15-29}
}
Intraclonal heterogeneity of genetically modified mammalian cells has been observed as a phenomenon that has a strong impact on overall transgene expression levels and that limits the predictability of transgene expression in genetically modified cells, thereby hampering single cell based screening approaches. The underlying mechanism(s) leading to this variance are poorly understood. To study the dynamics and mechanisms of heterogeneity of early stage silencing we analyzed the expression in… CONTINUE READING